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Comparison of soluble collagen in acid and pepsin extracted from three species Kilka fish (family Clupeidae) of the Caspian Sea
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Abstract: (3959 Views) |
| Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were extracted from mixture tissues of three abundant species of kilka fish such as common kilka (Clupeonella cultriventris), Anchovy kilka (Clupeonella engruliformis) and big eye kilka (Clupeonella grimmi (in the Caspian Sea. The samples were collected from fish landing site (Amirabad harbor) in the Mazandaran province. The fish were caught nightly by conical lift and transported in ice to the laboratory for collagen extraction. Non-collagenous proteins and fat of 15g mixture tissue of different organs were isolated by 0.1M NaOH and 10% Butyl alcohol solutions, respectively. Collagen was extracted by acidic and enzymatic methods at 4℃. In the next stage, collagen precipitates with NaCl and purification to 0.1M acetic acid solution and distilled water. The results showed that collagen of Clupeonella grimmi has the highest value of 4.14%, Clupeonella cultriventris and Clupeonella engruliformis have 1.04%, 0.94% on a dry weight respectively. The SDS-PAGE pattern showed that the ASC and PSC produced from this three species have hetrotrimer structure and made of two chains; α1 and α2. The extracted collagen is considered type I. Therefore, kilka fish especially Clupeonella grimmi can be used as a source for industrial and medical procedures. According to the results, kilka fish can be a commercial source in the Caspian Sea |
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| Keywords: Protein, Collagen, Kilka fish, Caspian Sea. |
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Full-Text [PDF 67 kb]
(2053 Downloads)
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Type of Study: Research |
Subject:
Fisheries
Received: 2017/09/13 | Accepted: 2017/09/13 | Published: 2017/09/13
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