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Effects of dietary green tea extract and oxidized fish oil on stress responses in Sturgeon hybrid (Husohuso♂ × Acipenserruthenus ♀)
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Vian Ebrahimi   , Amir Parviz Salati , Hamid Mohamadi , Hossein Pasha Zanosi |
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Abstract: (4919 Views) |
| In this study effect of feeding with different levels of green tea extract (GTE) and fish oxidized lipid (OFO) on stress responses to acute air exposure in sturgeon hybrid (Husohuso ♂ × Acipenserruthenus ♀) was studied. Nine diets including control (0 mg/kg GTE and 0 mg/kg OFO), GT50 (50 mg/kg GTE), GT100(100 mg/kg GTE), OFO50 (50 mg/kg OFO), OFO100 (100 mg/kg OFO), GT50OFO50 (50 mg/kg GTE, 50% OFO), GT50OFO100 (50 mg/kg GTE, 100 mg/kg OFO), GT100OFO50 (100 mg/kg GTE, 50 mg/kg OFO) and GT100OFO100 (100 mg/kg GTE, 100 mg/kg OFO) were provided. Fish were acclimated to laboratory condition for 2 weeks before starting the feeding trial. Fish (initial mean weight, 212.6 ± 0.7 g) were stockedrandomly into 1000 L fiberglass tanks with 10 fish per each tank. Three replicate groups of fish were hand-fed to satiation three times a day (8:00, 14:00 and 20:00) for 6 weeks. At the end of feeding trial fish were exposed to air exposure for 1 minute as an acute stress. During recovery, samples were taken at times 1, 2, 5 and 12 h. For evaluating stress responses,serum cortisol, glucose and lactate levelswas assayed. The results showed that diets containing OFO had adverse effects on sturgeon hybrid stress responses, while dietary GTE improved stress response and had protective effects of stress responses in fish fed OFO (P< 0.05). Air exposure caused an increase in serum cortisol levelin all experimental groups which was more prominent in fish fed OFO and did not receive GTE (P< 0.05). In fish fed GTE cortisol value returned to control earlier in comparing to control group and fish fed OFO. A similar pattern was recorded for glucose. Our findings showed that 50 and 100 mg/kg GTE could attenuate acute stress responses dramatically in sturgeon hybrid and caused serum cortisol and glucose to return to their normal levelsfaster in comparison to control and fish fed OFO, so could be used in diet to decrease stress responses in fish fed OFO.Feeding with GTE also caused decrease in serum lactate levelsin fish fed OFO. |
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| Keywords: Cortisol, Glucose, Lactate, Green tea, Oxidized fish oil. |
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Full-Text [PDF 369 kb]
(1390 Downloads)
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Type of Study: Research |
Subject:
Fisheries
Received: 2016/03/28 | Accepted: 2016/03/28 | Published: 2016/03/28
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