Guppy, Sex Reversal Induction, Hormon therapy, Alpha Methyltestosterone, Population

Golchin Manshadi, Alireza; Tarahomi, Mohammad; Madadi Jaberi, Aref

Volume 17, Issue 3 (Autumn 2025) 3 2025, 17(3): 58-67 | Back to browse issues page

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Golchin Manshadi A, Tarahomi M, Madadi Jaberi A. Guppy, Sex Reversal Induction, Hormon therapy, Alpha Methyltestosterone, Population. 3 2025; 17 (3) :58-67
URL: http://jmb.ahvaz.iau.ir/article-1-1023-en.html

Guppy, Sex Reversal Induction, Hormon therapy, Alpha Methyltestosterone, Population

Alireza Golchin Manshadi

, Mohammad Tarahomi

, Aref Madadi Jaberi

Department of Aquatic Animal Health, Kaz. C., Islamic Azad University, Kazerun, Iran.

Abstract: (386 Views)

To evaluate the effect of the androgen hormone 17α-methyl testosterone on guppy fry, breeders were initially divided into two groups of ten in two aquariums. The fry were split into five groups named A (control), B, C, D, and E, with two replicates each, and fed for 60 days as follows to allow sufficient growth and the emergence of secondary sexual characteristics: Group A (control): feed with hormone-free 0.4 pellet from Biomar, Group B: pellet immersed with 2 mg per 100 g of feed containing the hormone, Group C: pellet immersed with 4 mg per 100 g of feed containing the hormone, Group D: pellet immersed with 6 mg per 100 g of feed containing the hormone and Group E: pellet immersed with 8 mg per 100 g of feed containing the hormone. The aim was to determine growth performance and the development of secondary sexual traits in the fry over a 60-day period. he results showed that with increasing concentrations of 17α-methyl testosterone, the male population among the fry increased significantly (P ≤ 0.05). Specifically, the proportion of males in the control group was 48.9% compared to 51.1% females, while in Group E (the highest hormone dose used) this proportion rose to 75.42% males and 24.57% females, i.e., more than three times the female population. Results regarding the effect of 17α-methyl testosterone on the survivorship of fry across the different groups indicated that this hormone did not have a significant effect on increasing or decreasing fry survivorship, and the observed changes were not statistically significant (P > 0.05). Morphological examination of the fry across the groups under study showed no deformities or abnormalities in any of the groups. Overall, it can be concluded that 8 mg per 100 g of feed of 17α-methyl testosterone can act as an effective factor for altering the sex ratio in guppy fish without causing particular adverse effects.

Keywords: Guppy (Poecilia reticulata), Sex Reversal Induction, Hormon therapy, Alpha Methyltestosterone.

Full-Text [PDF 782 kb] (197 Downloads)

Type of Study: Research | Subject: biology
Received: 2025/08/2 | Accepted: 2025/11/3 | Published: 2025/11/3