Molecular and morphological identification of the family “Cerithiidae” on rocky shores of the Northern Persian Gulf
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Abstract: (4047 Views) |
Cerithiidae is among the most dominant families of the marine Gastropods in the rocky shores of the Persian Gulf. There is no previous study in relation to molecular and phylogeny of Cerithiidae in the research area. The molecular identification of the Cerithiidae species were been studied in the rocky shores of the northern parts of the Persian Gulf (for the first time) during the years 2013 and 2014. After morphological identifications of the species, DNA extraction, amplifying partial of cytochrome oxidase I (COI) and 16S rRNA and sequencing procedure were done in the laboratory. There were 4 species (Cerithium scabridum، Cerithium caeruleum، Cerithium sp. and Rhinoclavis sinensis) identified morphologicaly in the researe area. In this study 5 COI and 2 16S sequencing belonging to the species were measured. Also, phylogeny analyses with drawing phylogeny trees of Maximum Likelihood and Bayesian were done using MEGA6 and BEAST softwares. Results showed that Cerithium scabridum and Cerithium caeruleum, which are different morphologicly, were very similar in molecular identification. However, about Rhinoclavis sinensis both molecular and morphological identification had a same result. The results also showed that all species in the recent research had a common ancestor. |
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Keywords: Gastropoda, Cerithiidae, Cytochrome oxidase I, Phylogeny, Rocky shores, Persian Gulf. |
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Type of Study: Research |
Subject:
Fisheries Received: 2016/11/26 | Accepted: 2016/11/26 | Published: 2016/11/26
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