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The comparison of different method cultures of harmful dinoflagelCochlodiniumpolykrikoides at laboratory condition
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Reza Ghorbani Vagheie   , Alireza Asadi , Akbar Paigozar |
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Abstract: (5442 Views) |
| Cochlodinium is a dinoflagel thatit’s bloom in the coastal water of worlds and caused to fish mortality.Cochlodiniumwas detected in the Persian Gulf since 2008.After purification of C. polykricoidesusing light, it culturedusing medium of f/2 (modified), Guilard (f/2-Si), TMRL, Conway and ESM. Cochlodiniumcultured in tubes, erlanmeyer with volume of 100-1000 ml and aquariums with the volume of 16 liters (each with 10 liter water) with three replicates in each treatment, in the water salinity of 32 ppt, water tempreture of 25 0C, light intensity of 5000 lux and 12 hours dark and 12 hours light.The maximum cell density reached to 3283000 cells/l in f/2 (modyfied) medium, 2683000 cells/l in TMRL medium, 6393000 cells in erlanmeyerwith 200 ml volume, 2833000 cells in erlanmeyer with 500 ml volumeand 3600000 cells in TMRL medium in aquarium. Cochlodinium culture with TMRL medium in in erlanmeyer with 100 ml, 3207000 cells/l, 200 ml 3040000 cells/l, 1000 ml 2325000 cells/l counted.The culture of Cochlodiniumin f/2 was possible in the aquarium butthe culture of Cochlodinium in aquarium was not suitable. In f/2 medium the mean ofCochlodiniumin in each treatment was 3600000 cell/lin aquarium. Cochlodinium count in TMRL medium of aquarium was 550000 cell/l. Unsuccessful culture of Cochlodiniumusing Conway medium andGyllard (f/2-Si) indicated that these mediums weren’t suitablefor Cochlodinium culture. Based on results Cochlodinium count decreased with the increase of erlanmeyer volume in ESM. But, in tubes and in erlanmeyerwith volum100 ml, it was almost, successful. We can conclude that Cochlodinium culture required to special condition and medium, that with f/2 (modyfied) medium was gained. |
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| Keywords: Cochlodiniumpolykricoides, Culture medium, Cell density, Laboratory condition, Persian Gulf. |
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Full-Text [PDF 42 kb]
(1426 Downloads)
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Type of Study: Research |
Subject:
Fisheries
Received: 2015/01/10 | Accepted: 2015/01/10 | Published: 2015/01/10
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