Assessing Three Key Auxins (IAA, IPA & IBA) in Iranian Macroalgae: A Screening Survey Approach for Sustainable Biofertilizer Development

Khaledi, Hoda; Seyed Hashtroudi, Mehri; Mehdinia, Ali; Ghaderiardakani, Fatemeh; Zamani Jamshidi, Mohammad Mehdi

Volume 17, Issue 2 (Summer 2025) 3 2025, 17(2): 17-34 | Back to browse issues page

Mendeley

Zotero

RefWorks

Khaledi H, Seyed Hashtroudi M, Mehdinia A, Ghaderiardakani F, Zamani Jamshidi M M. Assessing Three Key Auxins (IAA, IPA & IBA) in Iranian Macroalgae: A Screening Survey Approach for Sustainable Biofertilizer Development. 3 2025; 17 (2) :17-34
URL: http://jmb.ahvaz.iau.ir/article-1-1029-en.html

Assessing Three Key Auxins (IAA, IPA & IBA) in Iranian Macroalgae: A Screening Survey Approach for Sustainable Biofertilizer Development

Hoda Khaledi

, Mehri Seyed Hashtroudi

, Ali Mehdinia

, Fatemeh Ghaderiardakani

, Mohammad Mehdi Zamani Jamshidi

Department of Ocean Sciences Research Center, Iranian National Institute for Oceanography and Atmospheric Science, 1411813389, Tehran, Iran

Abstract: (223 Views)

The increased use of chemical fertilizers to enhance the yield of agricultural products due to the increasing demand for food, the excessive use of these fertilizers, and the pollution caused by them, has led to the need to replace chemical fertilizers with environmentally friendly biological fertilizers, especially types of macroalgae. Continuing our research on the auxin contents of various Iranian macroalgae, we conducted a screening study to identify these macroalgae with the highest auxin concentrations, making them potential plant bio-stimulants. Chromatographic separation was performed using an Agilent 1100 HPLC system with UV and fluorescence detectors. The system was controlled, and data were processed using Agilent LC Chemstation software. Separation of the three auxins was achieved using an Extend-C18 column (5 μm, 4.6×250 mm; Agilent, USA). Limit of detection (LOD), and limit of quantification (LOQ) for each of the three standards were determined using the related equations. Auxins were detected in 14 samples, with IAA concentrations ranging from 0.43-13.04 ng/g (dry weight), IPA from 2-113 ng/g DW, and IBA from 1.99-7.97 ng/g DW. The highest auxin content was found in the brown algae Nizamuddin zanardini, consistent with previously reported studies. The results of this study have shown brown algae to be more promising sources for extracting auxins.

Keywords: Macroalgae, Auxins, Biofertilizer, HPLC analysis, Southern Iranian Coastal Areas

Full-Text [PDF 977 kb] (138 Downloads)

Type of Study: Research | Subject: biology
Received: 2025/07/19 | Accepted: 2025/06/28 | Published: 2025/06/28