Journal of Marine Biology

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

---

In this study, the effect of dried algae Chlorella sp. and two fresh algae Nannochloropsis oculta and Isochrysis galbana with high (5×10⁶ cell.ml^-1) and low (2.5×10⁶ cell.ml^-1) concentrations on reproductive indices (egg-bearing females, total eggs and Egg/total female (E/N)) of marine rotifer, Brachionus plicatilis were investigated. Rotifers were cultured in standard conditions in 500 ml plastic containers with initial density of 30 ind.ml^-1. The samplings of rotifers were conducted in the exponential phase (10 days) of population growth for counting the egg-bearing females and total eggs. Based on the two-way ANOVA, in day 1-2, the type of algae showed a significant effect at different concentrations and interactions had no significant different. From the third day to the sixth day (population peak), the interaction between algae and different concentration showed a significant effect on egg-bearing females and total eggs. Based on the results, the maximum number of egg-bearing females (122.23±7.21 ind.ml^-1) were observed on the fifth day in rotifers fed at high and low concentration of I. galbana (P<0.05). Also Maximum number of eggs (204±6.8 ind.ml^-1) were observed on the fourth day in rotifers fed at high concentration of I. galbana that was significantly higher than other treatments (P<0.05).  However, the lowest number of eggs significantly on the fifth day trial for both high and low concentrations of Chlorella sp. was obtained (P<0.05). In total number of eggs a high concentration of algae N.oculta and low concentrations of algae I. galbana did not show significant differences with each other (P>0.05). Also the maximum of E/N significantly was observed in both high and low concentrations of I. galbana. The study showed that dried algae, Chlorella  sp., in compare to two fresh algae I. galbana and N.oculta produce fewer egg-bearing females and despite the easy access the  dried Chlorella  sp.  is not a good alternative to fresh algae.

Keywords: : Brachionus plicatilis, Dried chlorella, Fresh algae, Egg-bearing females, Reproductive indices.

Full-Text [PDF 1087 kb]   (2674 Downloads)    

Add your comments about this article : Your username or Email:

---