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Effect of repetitive sperm stripping on some spermatology parameters, fertilization rate and the incubation trend in wild salmon Salmotruttacaspius, (Kessler 1877)
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Hossein Khara   , Somayeh Shamspour , Mostafa Rezvani , Mohaddeseh Ahmadnezhad , Mina Rahbar , SayedehSamaneh Mousavi |
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Abstract: (15427 Views) |
| This investigation was carried out at the ShahidBahonarKalardashtSalmonids Reproduction Center (KSRC)during spawning season during 2009. A total of 30 mature malesof wild salmon (Salmotruttacaspius) were used. In the first step all of male brooders (30 male) striped separatelyand 13 female striped eggs. Fertilization was made with mixing sperm and the eggs. After the eggs hardness process the eggs transferred to in a incubator, then the incubation process trends were evaluated. Eight days after the first step, the second step of sperm stripping occurred and 17 days after the second stage, third stage of sperm stripping was done. Similar to the first phase sperm stripping and fertilization was repeated and 25 male with 10 female for second stage and 15 male with 7 female for third stage. At the end of each three stages of sperm stripping, sperm density, spermatocrit percentage, sperm volume, sperm motility and morphology of spermatozoa (percentage of abnormal spermatozoa) were recorded and investigated its effect on fertilization rate, eyed eggs, hatching rate and larvae survival during repetitive sperm extraction were measures. The results showed that there were significant differences between the volume, motility and sperm morphology during third phase of the study (P<0.05). The highest fertilization rate (96.29%), eye spot time rate (91.52%) and hatching rate (93.82%) observed in the first stage of sperm extraction and lowest observed in the third stage of sperm extraction. In this study, sperm density, spermatocrit, and survival rate until the absorption of yolk sack stagein all the three stages were not significantly different (P>0.05). |
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| Keywords: Salmotruttacaspious, Repetitive sperm stripping, Artificial Propagation, Fertilization rate. |
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Full-Text [PDF 524 kb]
(2413 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2014/01/20 | Accepted: 2014/01/20 | Published: 2014/01/20
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