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Bacterial flora in farmed Husohusobefore and after releasing tocages located in theCaspian Sea
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Jalil Jalilpour   , Alireza Shenavar Masouleh , Mehdi Alizadeh , Mehdi Maasoumzadeh , Soheil Bazari Moghadam , Mohammadreza Mehrabi |
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Abstract: (4514 Views) |
| Considering the significance of sturgeon rearing and producing the meat and caviar which are one of the most development programs in aquaculture, it is necessary to investigate on hygiene condition of rearing environment to gather information about current rearing condition and to adopt the best method for prevention of disease and treatment in sturgeon. In this study, skin, gills, water and kidney bacteriaflora before transferring the farmed Husohuso(with average weight 103.45± 27.5 gr) and aftertransferringto the cage were determinedin fish washed with saline (0.85%NaCl) in the laboratoryunder sterile conditionssince 2011to 2012.Inorder tocounttheCFU (colony forming unit),1 square centimeter of skin, and 1 gram of fish gill were removedand washed for preparing of a homogeneous solutions of the decimal dilutions.In order to cultivate the kidney, the ventral surface of fish, disinfected and then the body aseptically opened for kidney sampling. Primary cultured on bacteriological medium was TSA. In addition to purification and identification of bacteria using standard bacterial identification, API 20E kit was used.Bacterial investigation results showed that total bacteria in rearing water tanks, fish gills and skin were 5.80-5.84Log cfuml-1, 3.28-3.41cfug-1 and 5.36-5.58 cfu (cm2)-1,respectively. The range of bacterial count insea water, skin and gill were 3.97-5.92 Log cfuml-1, 3.74-5.41 cfu (cm2)-1 and 2.01-3.40 cfug-1, respectively. Before and after releasingfish, isolated bacteria fromfish and rearing water were included Enterobacteriaceae,Aeromonassp., Pseudomonassp.,Acinetobactersp.,Staphylococcus, Halomunassp. andShewanellasp. |
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| Keywords: Farmed Husohuso, Caspian Sea, Total count, Bacteria |
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Full-Text [PDF 26 kb]
(1986 Downloads)
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Type of Study: Research |
Subject:
Fisheries
Received: 2015/01/14 | Accepted: 2015/01/14 | Published: 2015/01/14
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