Identification of Aeromonas caviae Bacterium with Lipase-Producing Capability from the Northern Persian Gulf (Optimization and Purification of the Enzyme)

Taghavi, Sara; Zekavati, Roya; Abbasi Montazeri, Effat; Roomiani, Laleh

Volume 16, Issue 4 (Winter 2025) 3 2025, 16(4): 55-69 | Back to browse issues page

Mendeley

Zotero

RefWorks

Taghavi S, Zekavati R, Abbasi Montazeri E, Roomiani L. Identification of Aeromonas caviae Bacterium with Lipase-Producing Capability from the Northern Persian Gulf (Optimization and Purification of the Enzyme). 3 2025; 16 (4) :55-69
URL: http://jmb.ahvaz.iau.ir/article-1-1034-en.html

Identification of Aeromonas caviae Bacterium with Lipase-Producing Capability from the Northern Persian Gulf (Optimization and Purification of the Enzyme)

Sara Taghavi

, Roya Zekavati

, Effat Abbasi Montazeri

, Laleh Roomiani

Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract: (330 Views)

In recent years, enzymes derived from aquatic microorganisms have attracted significant attention due to their unique characteristics, including stability under diverse industrial conditions. Accordingly, this study was conducted for the first time with the aim of identifying a bacterium capable of producing the industrially important enzyme lipase and evaluating its performance in the northern Persian Gulf, specifically in the Chouebdeh Port area of Abadan. Sampling was carried out from water and sediment in this region. In the initial screening, a culture medium containing a specific substrate was used to isolate lipase-producing bacteria, followed by identification of the target strain using 16S rRNA gene sequencing. Enzyme production under different conditions, including incubation time, temperature, pH, and carbon and nitrogen sources, was optimized first using the One Factor at a Time method and subsequently by the Plackett–Burman design. Finally, enzyme purification was performed using ammonium sulfate precipitation, dialysis, and gel filtration chromatography (Sephadex G-100). The lipase-producing bacterium with significant enzymatic activity (35 U/ml/min) after optimization was identified as Aeromonas caviae strain Persiangulf TA14. Optimal conditions for the activity of the lipase enzyme were observed on the first day of incubation (20 hours) at 37°C, pH 7, in a culture medium containing olive oil and peptone. The most influential factors were olive oil concentration and pH, respectively. The purified enzyme exhibited high specific activity (23.88 U/mg) and a purification fold of 41.7. The results of this study demonstrated that the lipase enzyme extracted from Aeromonas caviae possesses considerable enzymatic activity. Therefore, due to its resistant and efficient enzymes, particularly for applications in the food and biotechnology industries, this bacterium can be considered a suitable source for industrial lipase production.

Keywords: Aeromonas caviae, lipase enzyme, Persian Gulf.

Full-Text [PDF 1088 kb] (268 Downloads)

Type of Study: Research | Subject: biology
Received: 2024/10/25 | Accepted: 2025/02/4 | Published: 2025/02/12