fa تنوع ژنتیکی ماهی صبور )((Hamilton, 1822 Tenualosa ilisha) در خلیج‌فارس با استفاده از نشانگرهای AFLP شیلات Fisheries پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">ماهی صبور (</span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Tenualosa ilisha</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">یک‌گونه </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">سطح زی دریایی است که برای تخم&shy;ریزی به رودخانه</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> کوچ می&shy;کند. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">به‌منظور بررسی تنوع، تمایز و ساختار جمعیت ماهی صبور با استفاده از نشانگر </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">چند&shy;شکلی طولی قطعه تکثیرشده (</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Amplified Fragment Length Polymorphism</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">در آب‌های شمال خلیج‌فارس از مناطق آبادان، دیلم و بوشهر، از بافت باله دمی درمجموع 2</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">5</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">ماهی در خردادماه 1399 نمونه‌برداری گردید</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">سپس نمونه‌های موردبررسی در اتانول </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">96 درصد </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">فیکس شده و به آزمایشگاه انتقال یافت</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. پس از </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">استخراج</span></span></span></span> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">DNA</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">از بافت&shy;ها به روش فنل کلروفرم، در بررسی تنوع ژنتیکی با روش </span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">AFLP</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">،</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> از </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">پنج </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">ترکیب پرایمری </span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">MseI/EcorI</span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> استفاده شد</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">سپس آنالیز ژنتیکی با استفاده از نرم&shy;افزارهای </span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">GeneAlex</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">،</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"> Popgen</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">و </span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">TFPGA</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> انجام شد</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">.</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> این پنج ترکیب پرایمری مجموعاً</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> 2</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">6</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">3</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">باند قابل امتیازدهی و </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">3</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">2 </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">باند پلی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">&shy;</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">مورف تولید کردند</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">آنالیز داده</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">&shy;</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">ها نشان داد که میانگین درصد لوکوس&shy;های پلی</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">&shy;</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">مورفیسم در بین سه جمعیت ماهیان </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">صبور </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">99/11</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> درصد</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">، میانگین تنوع ژنتیکی </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">029/285</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">&plusmn;</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">0/0</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">و میانگین شاخص شانون </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">429/0 </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بود که نشان از تنوع ژنتیکی جمعیت‌ها داشت</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">نتایج آنالیز واریانس مولکولی </span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">(</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">AMOVA</span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> در سطح 99 درصد اختلاف بین سه جمعیت را</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> 20</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> درصد </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">و درون جمعیت‌ها را </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">80</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> درصد </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">نشان داد</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بر اساس تجزیه‌وتحلیل مؤلفه </span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">PCA</span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> و </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">دندروگرام فاصله ژنتیکی (</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">UPGMA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">مشخص گردید که میزان تفکیک جمعیت‌ها بر اساس فاصله جغرافیایی آن‌ها از یکدیگر بوده است</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">به‌طوری‌که نمونه</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">&shy;</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">های آبادان و دیلم کمترین فاصله ژنتیکی و نمونه&shy;های بوشهر و آبادان بیشترین فاصله ژنتیکی را دارند</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">آنچه از مطالعه حاضر می‌توان برآورد نمود این است که جمعیت&shy;های مجزایی از این ماهی در خلیج&shy;فارس وجود دارد.</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black"></span></span></span></span></span></span></span></span></div> <pre style="text-align:justify"> <span style="font-size:10pt"><span style="line-height:115%"><span courier="" new="" style="font-family:"><span style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Hilsa shad</span></span></span></span><span lang="EN" style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (<i>Tenualosa ilisha</i>) is a marine pelagic species that migrates to the river to spawn. In order to investigate the diversity, differentiation and population structure of </span></span></span></span><span style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">hilsa shad</span></span></span></span><span lang="EN" style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> using the Amplified Fragment Length Polymorphism </span></span></span></span><span style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(AFLP)</span></span></span></span><span lang="EN" style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> marker in the northern waters of the Persian Gulf from Abadan, Dilam and Bushehr regions, a total of 25 fish were sampled from the caudal fin tissue in June 2019 . Then the examined samples were fixed in 96% ethanol and transferred to the laboratory. After extracting DNA from tissues by phenol chloroform method, five MseI/EcorI primer combinations were used in genetic diversity analysis by AFLP method. Then genetic analysis was done using GeneAlex, Popgen and TFPGA software. </span></span></span></span><span style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">The percentage of polymorphic bands was 11.99%. Average of nei&rsquo;s genetic diversity was 0.285&plusmn;0.029, and average of shannon&rsquo;s index was 0.429. The results of AMOVA analysis indicated that 20% of the genetic variation contained within populations and 80%. </span></span></span></span><span lang="EN" style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Based on PCA component analysis and genetic distance dendrogram (UPGMA), it was determined that the degree of separation of populations was based on their geographical distance from each other. So that Abadan and Dilam samples have the lowest genetic distance and Bushehr and Abadan samples have the highest genetic distance. What can be estimated from the present study is that there are separate populations of this fish in the Persian Gulf.</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></pre> تنوع ژنتیکی, جمعیت, ماهی صبور, نشانگر AFLP, خلیج‌فارس Genetic variation, Population, Hilsa shad, AFLP marker, Persian Gulf. 45 54 http://jmb.ahvaz.iau.ir/browse.php?a_code=A-10-691-2&slc_lang=fa&sid=1 Hakimeh 2 and Fekrandish حکیمه فکراندیش 10031947532846006753 10031947532846006753 Yes Abdolrahim Pazira عبدالرحیم پذیرا 10031947532846006754 10031947532846006754 No Hossein Maheri حسین ماهری 10031947532846006755 10031947532846006755 No