Arash Shakouri, Omoleila Javanmard Kamy Ghazy Mahalleh, Fariborz Soheili,
Volume 7, Issue 1 (5-2015)
Abstract
Echinoderms, a group of marine invertebrates, are a rich source of antibacterial
compounds with high activity mechanism. The purpose of
the present study was, the determination of antibacterial effect using
different Extractants from sea urchin, Echinometra mathaei, on some
pathogenic bacterial strains in Chabahar in 1393. After sampling
and transport of samples to the laboratory, animals were dissected,
and Organs Shell, Spines, Gonad were Separated. After washing by
freeze-dryer samples were dried and then were milled, Extraced using
ethyl acetate, chloroform and methanol. The extracts were tested for
activity against Staphylococcus aureus, Enterococcus faecalis، Escherichia
coli and Klebsiella pneumonia. Disc diffusion and dilution the
culture medium methods were used for test hypothesis. Analysis of
the results was performed by One-way ANOVA. The results showed
that the antibacterial effect of different extracts of various organs are
significantly different (P <0.05). The results of the test Disc diffusion
agar showed the most antibacterial effect of the chloroform extract
Spines and Shell on gram negative bacteria E.coli. The test results
Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal
Concentration (MBC) Determined that chloroform Extracts of the
ethyl acetate extracts are better antibacterial effect. The chloroform
extract of Spines and shell most antibacterial effect showed the bacteria
E.coli organs Spines and Shell Echinometra mathaei, have significant
antibacterial activity that requires further study and purification
of the active components. The findings suggest that these compounds
of sea urchin could serve as a potential source of new antibacterial
substances to replace chemical agents.
