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The study on the occurrence of Vibrio spp. in oyster of Persian Gulf using Multiplex-PCR
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Mehdi Raissy   , Manouchehr Moumeni , Abbas Matin far , Hassan Momtaz , Firooz Fadaei fard |
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Abstract: (6268 Views) |
| Many species of Vibrio which is inhibited in warm waters are potentially regarded as food-borne pathogen for human. Aquatic animals and aquatic products can transmit the disease but there is no report about studying the contamination of oysters in Iran. The aim of this research was to study the occurrence of Vibrio species in oysters which caught from the Persian Gulf by using Multiplex PCR methods. In this study for the first time, edible parts of 60 oysters were studied by bacteriologic methods. Standard biochemical tests were done for identification of Vibrio isolates after culture in special mediums. Multiplex-Polymerase chain reaction (PCR) was used to confirm the results. The results showed that 18.3% of the studied samples (11 samples) contain Vibrio and no bacteria were found in 81.7% (49 samples). Among the Vibrio isolates, Vibrio harveyi was the species most frequently detected (10%) and V. parahaemolyticus and V. vulnificus were found in 3.3% and 5% of the samples, respectively. The results indicate that oyster of the Persian Gulf contain Vibrio species which are important for the human health. |
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| Keywords: Vibrio, Shellfish, Persian Gulf, Multiplex-PCR |
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Full-Text [PDF 498 kb]
(2518 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2014/01/21 | Accepted: 2014/01/21 | Published: 2014/01/21
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