fa بررسی اثر مهاری ضد باکتریایی و ضد قارچی عصاره اسفنج دریایی Haliclona Caerulea در دو فصل تابستان و زمستان منطقه جزرومدی جزایر لاوان و لارک، خلیج‌فارس شیلات Fisheries پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">این پژوهش به‌منظور بررسی خواص ضد باکتریایی و ضد قارچ عصاره‌های اسفنج </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Haliclona</span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"> c<i>aerulea</i></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">انجام شد. چندین کلونی اسفنج در فصل زمستان (اواسط اسفندماه 1395) و تابستان (اواسط مردادماه 1396) از عمق 15 تا 20 متری جزیره لاوان و لارک در خلیج‌فارس توسط غواص جمع‌آوری شد و عصاره گیری توسط حلال‌های آلی کلروفرم و متانول با استفاده از روش‌های استاندارد صورت گرفت. سپس فعالیت ضد قارچی و ضد باکتریایی کل عصاره‌ها به ترتیب با استفاده از روش ماکرودایلوشن بر روی سویه‌های قارچ و مخمر </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Candida albicans</span></span></i> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">ATcc</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">10231</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> و </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Aspergillus fumigatus</span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"> pTCC25009</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> و </span><a name="_Hlk104113494"><span style="color:#000000;">روش ماکرودایلوشن براث</span></a><span style="color:black"><a name="_Hlk104113494"> </a>بر سویه‌های باکتری </span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">15224</span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Escherichia coli </span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">ATCC</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">، </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Pseudomonas aeruginosa </span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">ATCC 27853</span></span><i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">، </span></span></span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">1609</span></span><i> </i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">PTCC</span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Salmonella typhi</span></span></i><span lang="AR-SA" new="" roman="" style="font-family:" times=""><span style="color:black">، </span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">ATCC 1715</span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Bacillus cereus</span></span></i><span lang="AR-SA" new="" roman="" style="font-family:" times=""><span style="color:black">، </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Staphylococcusaureus aureus </span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">ATCC 1764</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">و 1715</span></span></span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Bacillus subtilis spizizeni </span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">PTCC</span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">آزمایش و مورد مقایسه قرار گرفت </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">و سپس بر اساس آنالیز </span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">ANOVA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> و </span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">t-test</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> عملکرد آن‌ها </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">تعیین گردید.</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">بر اساس نتایج آنالیز آماری عملکرد ضد باکتریایی و ضد قارچی عصاره‌های کلروفرمی_ متانولی در دو فصل زمستان و تابستان در جزایر لاوان و لارک در غلظت‌های مختلف دارای سطح معنی‌دار با یکدیگر بودند (05/0</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">) و مشخص گردید مؤثرترین عملکرد ضد باکتریایی مربوط به عصاره فصل زمستان جزیره لارک و لاوان بر روی باکتری‌های </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">B.cereus</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">و </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">B.subtilis</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> بود. همچنین مؤثرترین عملکرد ضد قارچی بر روی مخمر </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">C.albicans</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">مربوط به عصاره فصل تابستان لارک و زمستان لاوان تعیین گردید.</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">نتایج</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> این تحقیق نشان داد که یک نمونه اسفنج </span></span></span></span><a name="_Hlk104116734"><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Haliclona caerulea</span></span></i></a> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">مربوط به دو ناحیه متفاوت اکولوژیک جزیره لاوان و لارک، در دو فصل زمستان و تابستان، خواص بیولوژیک متفاوتی را با توجه به منطقه زیست متفاوت بوم‌شناختی خود و فصول مختلف نشان می‌دهند، </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">همچنین باقابلیت اثر ضد قارچی و ضد باکتریایی و با مطالعات تکمیلی کلینیکی می‌توان به ساخت داروهای ضد قارچ و ضد باکتریایی ازاین‌گونه اسفنج مبادرت ورزید.</span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span new="" roman="" style="font-family:" times=""><span style="color:black">This research was conducted in order to investigate antibacterial and antifungal activity of <a name="_Hlk123652646"><i>Haliclona caerulea</i> </a>sponge extracts.</span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Several colonies of </span></span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">sponge were collected by divers at a depth of 15 to 20 meters in Lavan and Larak islands in the Persian Gulf in winter (Mid of March 2016) and summer (Mid of August 2017), and extraction was done using organic solvents chloroform and methanol using standard methods. Then, the antifungal and antibacterial activity of the whole extracts, respectively, using microdilution broth method on <i>Candida albicans</i> </span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">ATcc10231 and <i>Aspergillus fumigatus</i> pTCC25009 and broth method on </span></span><i><span new="" roman="" style="font-family:" times=""><span style="color:black">Escherichia coli </span></span></i><span new="" roman="" style="font-family:" times=""><span style="color:black">ATCC<i></i>¹ 15224, <i>Pseudomonas aeruginosa </i>ATCC 27853, <i>Salmonella typhi</i> PTCC 1609, <i>Bacillus cereus </i>ATCC 1715, <i>Staphylococcusaureus aureus </i>ATCC 1764 and </span></span><i><span new="" roman="" style="font-family:" times=""><span style="color:black">Bacillus subtilis spizizeni&nbsp; </span></span></i><span new="" roman="" style="font-family:" times=""><span style="color:black">PTCC 1715 were tested and compared and then their performance was determined based on ANOVA analysis and t-test. Based on the results of statistical analysis, the antibacterial and antifungal performance of chloroform-methanolic extracts in winter and summer in Lavan and Larak island in different concentrations had a significant level with each other (P<0.05) and it was determined that the most effective performance the antibacterial effect of Larak and Lavan island winter extract was on <i>B.cereus</i> and <i>B.subtilis</i> bacteria. Also, the most effective antifungal activity on <i>C.albicans</i> yeast was determined from the extract of summer Larak and winter Lavan.</span></span><b> </b><span new="" roman="" style="font-family:" times=""><span style="color:black">The results of this research showed that a sample of </span></span><i><span new="" roman="" style="font-family:" times=""><span style="color:black">Haliclona caerulea </span></span></i><span new="" roman="" style="font-family:" times=""><span style="color:black">sponge related to two different ecological regions of Lavan and Larak islands, in winter and summer, has different biological properties according to its different ecological bioregion and different seasons. They show, also with the ability of antifungal and antibacterial effect and with additional clinical studies, it is possible to make antifungal and antibacterial drugs from this type of sponge.</span></span><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></b></span></span></span></div> عصاره‌های اسفنج, ضد قارچ, ضد باکتریایی, جزیره لاوان, جزیره لارک, خلیج‌فارس. Sponge extracts, Antifungal, Antibacterial, Lavan Island, Larak Island, Persian Gulf. 45 58 http://jmb.ahvaz.iau.ir/browse.php?a_code=A-10-652-7&slc_lang=fa&sid=1 Elnaz Shafeian الناز شافعیان 10031947532846006837 10031947532846006837 No Pargol Ghavam mostafavi پرگل قوام مصطغوی 10031947532846006838 10031947532846006838 Yes Mehdi Moridi farimani مهدی مریدی فریمانی 10031947532846006839 10031947532846006839 No Ali Mashinchian Moradi علی ماشینچیان مرادی 10031947532846006840 10031947532846006840 No Melika Nazemi ملیکا ناظمی 10031947532846006841 10031947532846006841 No