Marine Biology
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3
Basic Sciences
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jalali
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2021
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مقایسه ساختار ژنتیکی مولدین میگوی سفید غربی (Litopenaeus vannamei Boone, 1931) وارداتی و پرورشی با استفاده از روش ریز ماهوارهای
Comparison of genetic structure of Litopenaeus vannamei (Boone, 1931) broodstocks imported and farmed by microsatellite method
شیلات
Fisheries
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">هدف از اجرای این تحقیق مقایسه شاخصهای ژنتیکی ذخیرههای مختلف مولدین میگوی سفید غربی </span></span></span></span><span lang="FA" style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">(</span></span></span><i><span dir="LTR" style="color:black">Litopenaeus vannamei</span></i><span lang="FA" style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">وارداتی (دو ذخیره) با مولدین پرورشی (یک ذخیره) مراکز تکثیر استان بوشهر در سال 1400 بود. در این مطالعه پس از استخراج </span></span></span></span><span dir="LTR" style="color:black">DNA</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> از بافت عضله مولدین ذخیرههای مختلف، شاخصهای ژنتیکی آنها با استفاده 10 جفت </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">نشانگر اختصاصی پلی مورفیک</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> از طریق روش ریز ماهوارهای موردبررسی قرار گرفت. نتایج نشان داد که </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">میانگین آللهای واقعی در مولدین پرورشی نسل اول </span></span></span></span><span lang="FA" style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">(</span></span></span><span dir="LTR" style="color:black">F1</span><span lang="FA" style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">با میزان 298/0</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">000/4 بیشتر از مقادیر مربوط به مولدین وارداتی نسل صفر در سال اول و دوم </span></span></span></span><span lang="FA" style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">(</span></span></span><span dir="LTR" style="color:black">F0:1, 2</span><span lang="FA" style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">به ترتیب با میزان 269/0</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">500/3 و 233/0</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">900/3 میباشد</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">. </span></span></span></span><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">میانگین هتروزیگوسیتی مشاهدهشده در مولدین پرورشی با میزان 081/0</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">259/0 نسبت به مولدین وارداتی نسل صفر سال اول و دوم</span></span></span></span></em><em new="" roman="" style="font-family:" times=""> </em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">به ترتیب با میزان 075/0</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">240/0 و 040/0</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">161/0 بیشتر بود، لیکن با توجه به افزایش هتروزیگوسیتی </span></span></span></span></em><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">قابلانتظار میزان تنوع ژنتیکی در هر سه ذخیره </span></span></span></span><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">کاهش یافت</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">. ضریب همخونی در مولدین وارداتی نسل صفر سال دوم با میزان 276/0</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">480/0 بیشتر از مقادیر بهدستآمده در مولدین پرورشی و وارداتی نسل صفر سال اول به ترتیب با میزان 456/0</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">303/0 و 286/0</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">300/0 بود. </span></span></span></span></em><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بیشترین میزان فاصله ژنتیکی (380/0) و تمایز ژنی (100/0) میان مولدین وارداتی نسل صفر سال اول با نسل صفر وارداتی سال دوم وجود داشت. </span></span></span></span><em new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">بر اساس نتایج بهدستآمده یکی از دلایل کاهش شاخصهای ژنتیک در مولدین وارداتی در مقایسه با مولدین پرورشی نسل اول میتواند ناشی از کم بودن تعداد مولدین وارداتی مشارکتکننده در آمیزش (جمعیت بنیادی) و بهبود ژنتیکی مولدین پرورشی بوده باشد.</span></span></span></span></em><em new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="color:black"></span></span></span></em></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="unicode-bidi:embed"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">This study aimed to compare the genetic characteristics of different stocks of <i>Litopenaeus</i> <i>vannamei</i> imported broodstocks two stocks (F0: 1 and 2) with reared one stock (F1) in Iranian climate in during 2021. After extracting DNA from the muscle tissue of different stocks, their genetic index has been examined using ten pairs of specific polymorphic microsatellite markers. Results showed that the mean of alleles in F1 broodstock with a rate of 4.000 ±0.298 was higher than the values of F0: 1 and 2 broodstock (3.500±0.269 and 3.900±0.233, respectively).</span> <span style="color:black">The average heterozygosity observed in F1 with a rate of 0.259±0.081 was more than imported zero broodstock (F0: 1 and 2) with a rate of 0.240±0.075 and 0.161±0.040, respectively, increasing in expected heterozygosity had reduced genetic diversity in all three stocks. The inbreeding coefficient in the imported broodstocks of the second year of the zero generation (0.480±0.276) was higher than the values obtained in the cultured (0.303±0.456) and imported broodstocks of the first year of the zero generation (0.300±0.286). There was the greatest genetic distance (0.380) and genetic differentiation (0.100) between the imported broodstocks of the first year of the zero generation and the broodstocks of the second year of the imported zero generation. According to the results, one of the reasons for the decrease in genetic indicators in imported broodstocks compared to reared broodstocks can be due to the low number of imported broodstocks participating in mating (fundamental population) and genetic improvement of cultured broodstocks.</span></span></span></span></span></div>
میگوی سفید غربی, مولد وارداتی, مولد پرورشی, شاخص ژنتیک.
Litopenaeus vannamei, imported broodstock, reared broodstock, Genetic index.
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64
http://jmb.ahvaz.iau.ir/browse.php?a_code=A-10-237-8&slc_lang=fa&sid=1
Mohammad Khalil
Pazir
محمدخلیل
پذیر
10031947532846006542
10031947532846006542
Yes
Ayeh Sadat
Sadr
آیه سادات
صدر
10031947532846006543
10031947532846006543
No
Mohammad Ali
Nazari
محمدعلی
نظاری
10031947532846006544
10031947532846006544
No
Sajjad
Pourmozaffar
سجاد
پورمظفر
10031947532846006545
10031947532846006545
No
Ashkan
Ajdari
اشکان
اژدری
10031947532846006546
10031947532846006546
No
Aghil
Dashtian Nasab
10031947532846006547
10031947532846006547
No
Aghil
Dashtian Nasab
عقیل
دشتیان نسب
10031947532846006548
10031947532846006548
No