fa تعیین سطح غلظت فلزات سنگین مس، روی و آهن در بافت عضله ماهی صبیتی (Acanthopagrus cuvieri) در بندر بوشهر شیلات Fisheries پژوهشي Research <div style="text-align: justify;"><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">این مطالعه باهدف بررسی سطوح فلزات سنگین مس، روی و آهن در بافت عضله ماهی صبیتی (</span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">Acanthopagrus</span></span></span></em> <em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">cuvieri</span></span></span></em><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">) در بندر بوشهر انجام پذیرفت. بدین منظور تعداد 30 قطعه ماهی صبیتی (</span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">A.</span></span></span></em> <em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">cuvieri</span></span></span></em><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">) با میانگین وزنی 101/156</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">73/840 گرم و میانگین طولی 089/3</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">28/34 سانتی&shy;متر </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">در فصل بهار سال 1397 تهیه شد. نمونه&shy;ها را با آب مقطر شستشو داده و مقداری از بافت عضله خالص جداسازی گردید. بعد از خشک‌کردن رطوبت و آب موجود در فضای میان بافتی نمونه‌ها، به میزان 5/0 گرم نمونه بافتی پودر شده جدا و با 5 میلی‌لیتر اسید نیتریک غلیظ هضم اسیدی نمونه‌ها صورت گرفت. </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">برای اندازه&shy;گیری سطح فلزات سنگین </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">مس، روی و آهن</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> از </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">دستگاه جذب اتمی </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">مجهز به کوره گرافیتی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">Perkin Elmer 3030</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">استفاده شد. </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">بر اساس آزمایش‌های انجام‌شده میانگین غلظت فلزات مس، روی و آهن در بافت عضله ماهی صبیتی به ترتیب </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">22/0</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">32/6، 14/0</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">12/61 و 36/0</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">86/4 </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">میلی&shy;گرم در کیلوگرم وزن خشک محاسبه گردید. میزان فلزات سنگین مس و روی در بافت عضله ماهی </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">صبیتی </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">در بندر بوشهر پایین&shy;تر از حد مجاز استاندارد&shy;های سازمان بهداشت جهانی (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">WHO</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">)، سازمان جهانی غذا و کشاورزی (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">FAO</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">)، مرکز ملی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">بهداشت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">پزشکی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">استرالیا (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">NHMRC</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">) و وزارت کشاورزی، شیلات و غذای انگلستان </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">UK</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">MAFF</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">)</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> بود؛ اما میزان فلز سنگین آهن بیشتر از مقدار مجاز اعلام‌شده توسط سازمان غذا و دارو آمریکا (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">FDA</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;">) اعلام شد. ازاین‌رو استفاده ازاین‌گونه برای مصارف انسانی مشکل چندانی را از دیدگاه سلامت و بهداشت عمومی ایجاد نخواهد کرد.</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:12.0pt;"></span></span></span></div> The present research was carried out with the aim of measuring the levels of the heavy metals Cu, Zn and Fe in the muscle tissues of the <em>Acanthopagrus cuvieri</em> in Bushehr port. To this end, 30 <em>A. cuvieri</em> with an average of 840.73&plusmn;156.101 gr and 34.28&plusmn;3.089 cm were hunted in spring 2018. The samples were washed with distilled water and a portion of the pure muscle tissues was isolated. After dehydrating the tissues and discharging the water in the intertextual space, 0.5 gr of the powdered tissue sample was isolated and the acid digestion of the samples was carried out using 5 ml of concentrated nitric acid. To measure the levels of the heavy metals Cu, Zn and Fe a (Perkin Elmer 3030) atomic absorption spectrophotometer was employed. The results of measuring the concentrations of the heavy metals Cu, Zn and Fe in the muscle tissues of the <em>A. cuvieri</em> suggested that the mean and standard deviation were (6.32&plusmn;0.22, 61.12&plusmn;0.14 and 4.86&plusmn;0.36) mg/kg dry weight during spring, respectively. The results of this study revealed that the Cu and Zn concentration in muscle tissues of <em>A. cuvieri</em> in study station were lower than the levels permitted within the standards of World Health Organization (WHO), Food and Agricultural Organization (FAO), National Health and Medical Research Council (Australia) (NHMRC), Ministry of Agriculture and Forestry and Fisheries UK (MAFF). But Fe concentration in muscle tissues of Acanthopagrus cuvieri in study station was higher than the level permitted within the standard of Food and Drug Administration (FDA). Hence, these two species will not pose severe general health risks to human consumers. مس, روی, آهن, بندر بوشهر, Acanthopagrus cuvieri The present research was carried out with the aim of measuring the levels of the heavy metals Cu, Zn and Fe in the muscle tissues of the Acanthopagrus cuvieri in Bushehr port. To this end, 30 A. cuvieri with an average of 840.73±156.101 gr and 34.28±3.0 35 42 http://jmb.ahvaz.iau.ir/browse.php?a_code=A-10-274-4&slc_lang=fa&sid=1 Abdol Hossein Doulah عبدالحسن دولاح 10031947532846005414 10031947532846005414 Yes Cellular and Molecular Biology Department, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Razagh Obeidi رزاق عبیدی 10031947532846005415 10031947532846005415 No Young Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, Iran