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Inthis study, the effects oflutein (a plant pigment) on thegrowth performance, nutrition, survival rate and pigmentation of Salmotruttacaspiuswith initial average weight 87±0.37gwere studiedfor 8 weeks. 4treatmentswith 3replicatesweretestedas follows: T1: control, T2: 14 mg/kg lutein, T3: 16 mg/kg lutein, T4: 20 mg/kg lutein. The results showed that there were no significantdifferencebetweentheweightandlength of the treatments which fed lutein) P<0.05(, by the means these treatments had significantly more weight and length than the control (P<0.05). In growth performance, nutritional parameters and the survival rate between the treatments which fed lutein, no significant difference were observed, except in the condition factor (CF) )P<0.05(. But these treatments showed significant differences compared with control in all of these factors except in the FCR)P<0.05(. The minimum range of food conversion ratio (FCR) and CF, and the maximum range of body weight index (BWI) and growth rate (GR) were observed in T4. In terms ofpigmentationbetween experimentaltreatments,lutein20 mg/kg was created more color change than othertreatments andcontrol. Theresultsshowed thatthe usage oflutein in the dietwaseffective forSalmotruttacaspius.

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In this study, the effects of water soluble fraction (WSF) of crude oil on liver tissue of fingerlings beluga(Husohuso) with mean weight 10.76 ± 0.23g was assessed in the Caspian Sea. Firstly, the LC5096hvalue inwater soluble fraction of crude oil was 46.68 mgL-1. Control group and 3 other treatments (atconcentrations 0.4LC50,0.6LC50,and 0.8LC50) were lower than the LC5096h. Each experimental group had 3 replicates including30 fish and experiment duration was 96h. Physical and chemical factors of water were measured and sampling of liver was doneeach 24h. According to the results, different concentrations of water soluble fraction of crude oil (WSF) showed high damages in liver tissue even from the first time of experiment. Several damages such as hypertrophy and congestion of sinusoids, hypertrophy of hepatocyte, vacoulation degeneration and fatty degeneration, bile stagnant, necrosis, cirrhosis and fibrosis were observed in samples. Results showed that in high concentrations of crude oil or more residence of fish in this pollution, unreturnable and serious damages were occurred. Results showed that even exposing to 0.4LC50WSF ofthe Caspian Sea crude oil can lead to serious damages in liver tissue (fatty degeneration, karyorrhexis, karyolysis, and necrosis). So exposing to 0.4LC50 can cause to health risk in beluga fingerlings and probability of the mortality will be increasedwith the increase of exposureduration.