|
Introduction of genetic markers suitable for identification and isolation of Scenedesmus microalgae species
|
| |
|
|
|
Abstract: (408 Views) |
The unicellular alga scenedesmus is green algae of great industrial and nutritional value. Therefore, further identification of this genus and its prolific species will be very useful. Usually, the identification of species of this microalga with different morphological and physiological methods has not been very successful, because these traits can change under different growth conditions and cause confusion in the classification of this alga. Therefore, in this study, molecular markers were used to better and more accurately identify this alga, and the genetic diversity of some strains of scenedesmus algae in Aras River was investigated using molecular markers. In this study, 2 samples of scenedesmus algae related to Aras river and ornamental fish breeding place in Tabriz were DNA extracted and polymerase chain reaction was performed using specific primers of ITS, tufA, 18s rRNA, CV genes, following sequencing of PCR products. DNA sequences were performed and analyzed. The phylogenetic tree related to markers was drawn into three models by Mega7 software and the trees were compared and based on the results, the tufA marker was selected as the appropriate marker. By examining the phylogenetic tree related to the tufA marker, the studied sample of scenedesmus related to Aras River and ornamental fish breeding place in Tabriz was placed in a cluster with S. alcuminatus algae which was used as a control and has a close relationship. This confirms the morphological similarity of these algae. The use of this marker seems to be more accurate compared to 18s rRNA, CV and ITS markers. |
|
| Keywords: Scenedesmus, morphological identification, molecular marker, Aras River. |
|
|
Full-Text [PDF 94 kb]
(1162 Downloads)
|
Type of Study: Research |
Subject:
Fisheries
Received: 2023/01/15 | Accepted: 2021/09/1 | Published: 2021/09/1
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
